✉ corresponding author | * contributed equally | lab members in bold
2025
50. Engineering encapsulin nanocages for drug delivery
Kwon S, Giessen TW✉ (submitted)
49. HIV-1 binds dynein directly to hijack microtubule transport machinery
Badieyan S, Lichon D, Andreas MP, Gillies JP, Peng W, DeSantis ME, Aiken T, Böcking CR, Giessen TW, Campbell EM, Cianfrocco MA✉
Science Advances (2025) | bioRxiv preprint: 555335
48. Structural and biochemical characterization of a widespread enterobacterial peroxidase encapsulin
Ubilla-Rodriguez NC, Andreas MP, Giessen TW✉
Advanced Science (2025) | bioRxiv preprint: 625667
2024
47. The structural diversity of encapsulin shells
Giessen TW✉
ChemBioChem (2024)
46. Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance
Kwon S, Andreas MP, Giessen TW✉
ACS Nano (2024) | bioRxiv preprint: 592161
45. A two-component quasi-icosahedral protein nanocompartment with variable shell composition and irregular tiling
Dutcher CA, Andreas MP, Giessen TW✉
bioRxiv (2024)
44. The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell
Andreas MP, Giessen TW✉
Nature Communications (2024) | bioRxiv preprint: 590730
43. Structural and biochemical characterization of an encapsulin-associated rhodanese from Acinetobacter baumannii
Benisch R, Giessen TW✉
Protein Science (2024) | bioRxiv preprint: 581022
42. Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages
Szyszka T, Andreas MP, Lie F, Miller L, Adamson L, Fatehi F, Twarock R, Draper B, Jarrold MF, Giessen TW✉, Lau YH✉
PNAS (2024) | bioRxiv preprint: 579038
41. Cyclodipeptide oxidase is an enzyme filament
Andreas MP, Giessen TW✉
Nature Communications (2024) | bioRxiv preprint: 559410
40. Structural basis for peroxidase encapsulation inside the encapsulin from the Gram-negative pathogen Klebsiella pneumoniae
Jones JA*, Andreas MP*, Giessen TW✉
Nature Communications (2024) | bioRxiv preprint: 558302
39. A widespread bacterial protein compartment sequesters and stores elemental sulfur
Benisch R, Andreas MP, Giessen TW✉
Science Advances (2024) | bioRxiv preprint: 545984
2023
38. Structure and heterogeneity of a highly cargo-loaded encapsulin shell
Kwon S, Andreas MP, Giessen TW✉
Journal of Structural Biology (2023) | bioRxiv preprint: 550694
37. Encapsulin Cargo Loading: Progress and Potential
Jones JA, Benisch R, Giessen TW✉
Journal of Materials Chemistry B (2023)
36. Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage
Jones JA, Andreas MP, Giessen TW✉
Biomacromolecules (2023) | bioRxiv preprint: 499790
2022
35. Engineered protein nanocages for concurrent RNA and protein packaging in vivo
Kwon S, Giessen TW✉
ACS Synthetic Biology (2022) | bioRxiv preprint: 496435
34. Heterologous Expression and Purification of Encapsulins in Streptomyces coelicolor
Andreas MP, Giessen TW✉
MethodsX (2022)
33. Encapsulins
Giessen TW✉
Annual Review of Biochemistry (2022)
32. Direct reconstitution and study of SUN protein interactions in vitro using mammalian cell-free expression
Majumder S, Hsu YY, Andreas MP, Giessen TW, Liu AP✉
Biochemistry (2022) | bioRxiv preprint: 439350
31. Pore structure controls stability and molecular flux in engineered protein cages
Adamson LR*, Tasneem N*, Andreas MP*, Close W, Jenner EN, Szyszka TN, Young RD, Cheah LC, Norman A, MacDermott-Opeskin HI, O’Mara, ML, Sainsbury F, Giessen TW✉, Lau YH✉
Science Advances (2022) | bioRxiv preprint: 428512
2021
30. Triggered reversible disassembly of an engineered protein nanocage
Jones JA*, Cristie-David AS*, Andreas MP, Giessen TW✉
Angewandte Chemie International Edition (2021) | bioRxiv preprint: 440480
29. Large-scale computational discovery and analysis of virus-derived microbial nanocompartments
Andreas MP, Giessen TW✉
Nature Communications (2021) | bioRxiv preprint: 436031
28. Engineered Icosahedral Protein Cage as a Flexible Model System to Study Properties of Multi-Motor Transport of Viral Cargoes Badieyan S, Andreas MP, Giessen TW✉, Cianfrocco MW✉
Biophysical Journal 120 (3), 289a (2021)
27. Exploring in vivo cargo loading of encapsulin nanocompartments
Wiggert JA, Rollins N, Silver PA, Giessen TW✉
Scientific Reports (2021)
2020
26. Engineering spatiotemporal organization and dynamics in synthetic cells
Groaz A, Moghimianavval H, Tavella F, Giessen TW, Vecchiarelli AG, Yang Q, Liu AP✉
WIREs Nanomedicine and Nanobiotechnology (2020)
25. Advances in encapsulin nanocompartment biology and engineering
Jones JA, Giessen TW✉
Biotechnology & Bioengineering (2020)
2019
24. The discovery of six new genes encoding for encapsulin nanocompartments in metagenomes
Tracey J, Coronado M, Giessen TW, Lau, MCY, Silver PA, Ward B✉
Scientific Reports (2019)
23. Engineered inter-species amino acid cross-feeding increases population evenness in a synthetic bacterial consortium
Ziesack M, Gibson T, Oliver JKW, Shumaker AM, Hsu BB, Riglar DT, Giessen TW, DiBenedetto NV, Bry L, Way JC, Silver PA, Gerber GK✉ mSystems (2019) | bioRxiv preprint: 426171
22. Large protein organelles form a new iron sequestration system with high storage capacity
Giessen TW✉, Orlando BJ, Verdegaal AA, Chambers MG, Gardener J, Bell DC, Birrane G, Liao M✉, Silver PA✉
eLife (2019) - Highlighted in: eLife digest - Highlighted in: Cell | bioRxiv preprint: 511345
21. A synthetic system that senses Candida albicans and inhibits virulence factors
Tscherner M, Giessen TW, Markey L, Kumamoto CA, Silver PA✉
ACS Synthetic Biology (2019) | bioRxiv preprint: 342287
2018
20. Prokaryotic nanocompartments form synthetic organelles in a eukaryote
Lau YH*, Giessen TW*, Altenburg WJ, Silver PA✉
Nature Communications (2018) | bioRxiv preprint: 244095
2017
19. Engineering carbon fixation with artificial protein organelles
Giessen TW✉, Silver PA✉
Current Opinion in Biotechnology (2017)
18. Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation
Riglar DT, Giessen TW, Baym M, Kerns SJ, Niederhuber MF, Bronson RT, Kotula JW, Gerber GK, Way JC, Silver PA✉
Nature Biotechnology (2017)
17. Widespread distribution of encapsulin nanocompartments reveals functional diversity
Giessen TW, Silver PA✉
Nature Microbiology (2017) | bioRxiv preprint: 085266
2016
16. Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution
Liu X, Lopez P, Giessen TW, Giles M, Way J, Silver PA✉
Scientific Reports (2016) | bioRxiv preprint: 085233
15. Encapsulins: microbial nanocompartments with applications in biomedicine, nanobiotechnology and materials science
Giessen TW✉
Current Opinion in Chemical Biology (2016)
14. A catalytic nanoreactor based on in vivo encapsulation of multiple enzymes in an engineered protein nanocompartment
Giessen TW, Silver PA✉
ChemBioChem (2016)
13. Converting a natural protein compartment into a nanofactory for the size-constrained synthesis of antimicrobial silver nanoparticles Giessen TW, Silver PA✉
ACS Synthetic Biology (2016)
2015
12. Encapsulation as a strategy for the design of biological compartmentalization
Giessen TW, Silver PA✉
Journal of Molecular Biology (2015)
11. Rational and combinatorial tailoring of bioactive cyclic dipeptides
Giessen TW✉, Marahiel MA
Frontiers in Microbiology (2015)
10. A Synthetic Adenylation‐Domain‐Based tRNA‐Aminoacylation Catalyst
Giessen TW✉, Altegoer F, Nebel AJ, Steinbach RM, Bange G✉, Marahiel MA✉
Angewandte Chemie International Edition (2015)
2014
9. The tRNA-dependent biosynthesis of modified cyclic dipeptides
Giessen TW✉, Marahiel MA✉
International Journal of Molecular Sciences (2014)
2013
8. Insights into the generation of structural diversity in a tRNA-dependent pathway for highly modified bioactive cyclic dipeptides
Giessen TW, von Tesmar AM, Marahiel MA✉
Chemistry & Biology (2013)
7. A tRNA-dependent two-enzyme pathway for the generation of singly and doubly methylated ditryptophan 2, 5-diketopiperazines Giessen TW, von Tesmar AM, Marahiel MA✉
Biochemistry (2013)
6. Two [4Fe-4S] clusters containing radical SAM enzyme SkfB catalyze thioether bond formation during the maturation of the sporulation killing factor
Flühe L, Burghaus O, Wieckowski BM, Giessen TW, Linne U, Marahiel MA✉
Journal of the American Chemical Society (2013)
2012
5. Ribosome-independent biosynthesis of biologically active peptides: Application of synthetic biology to generate structural diversity Giessen TW, Marahiel MA✉
FEBS Letters (2012)
4. Isolation, Structure Elucidation, and Biosynthesis of an Unusual Hydroxamic Acid Ester-Containing Siderophore from Actinosynnema mirum
Giessen TW, Franke KB, Knappe TA, Kraas FI, Bosello M, Xie X, Linne U, Marahiel MA✉
Journal of Natural Products (2012)
3. An enzymatic pathway for the biosynthesis of the formylhydroxyornithine required for rhodochelin iron coordination
Bosello M, Mielcarek A, Giessen TW, Marahiel MA✉
Biochemistry (2012)
2. Exploring the mechanism of lipid transfer during biosynthesis of the acidic lipopeptide antibiotic CDA
Kraas FI, Giessen TW, Marahiel MA✉
FEBS Letters (2012)
2011
1. A four-enzyme pathway for 3, 5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin Giessen TW, Kraas FI, Marahiel MA✉
Biochemistry (2011)