✉ corresponding author | * contributed equally | lab members in bold
2024
47. Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance
Kwon S, Andreas MP, Giessen TW✉
bioRxiv (2024)
46. A two-component quasi-icosahedral protein nanocompartment with variable shell composition and irregular tiling
Dutcher CA, Andreas MP, Giessen TW✉
bioRxiv (2024)
45. The biosynthesis of the odorant 2-methylisoborneol is compartmentalized inside a protein shell
Andreas MP, Giessen TW✉
bioRxiv (2024)
44. Structural and biochemical characterization of an encapsulin-associated rhodanese from Acinetobacter baumannii
Benisch R, Giessen TW✉
bioRxiv (2024)
43. Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages
Szyszka T, Andreas MP, Lie F, Miller L, Adamson L, Fatehi F, Twarock R, Draper B, Jarrold MF, Giessen TW✉, Lau YH✉
PNAS (2024) | bioRxiv preprint: 579038
42. Cyclodipeptide oxidase is an enzyme filament
Andreas MP, Giessen TW✉
Nature Communications (2024) | bioRxiv preprint: 559410
41. Structural basis for peroxidase encapsulation inside the encapsulin from the Gram-negative pathogen Klebsiella pneumoniae
Jones JA*, Andreas MP*, Giessen TW✉
Nature Communications (2024) | bioRxiv preprint: 558302
40. HIV-1 binds dynein directly to hijack microtubule transport machinery
Badieyan S, Lichon D, Andreas MP, Gillies JP, Peng W, Shi J, DeSantis ME, Aiken T, Böcking CR, Giessen TW, Campbell EM, Cianfrocco MA✉
bioRxiv (2023)
39. A widespread bacterial protein compartment sequesters and stores elemental sulfur
Benisch R, Andreas MP, Giessen TW✉
Science Advances (2024) | bioRxiv preprint: 545984
2023
38. Structure and heterogeneity of a highly cargo-loaded encapsulin shell
Kwon S, Andreas MP, Giessen TW✉
Journal of Structural Biology (2023) | bioRxiv preprint: 550694
37. Encapsulin Cargo Loading: Progress and Potential
Jones JA, Benisch R, Giessen TW✉
Journal of Materials Chemistry B (2023)
36. Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage
Jones JA, Andreas MP, Giessen TW✉
Biomacromolecules (2023) | bioRxiv preprint: 499790
2022
35. Engineered protein nanocages for concurrent RNA and protein packaging in vivo
Kwon S, Giessen TW✉
ACS Synthetic Biology (2022) | bioRxiv preprint: 496435
34. Heterologous Expression and Purification of Encapsulins in Streptomyces coelicolor
Andreas MP, Giessen TW✉
MethodsX (2022)
33. Encapsulins
Giessen TW✉
Annual Review of Biochemistry (2022)
32. Direct reconstitution and study of SUN protein interactions in vitro using mammalian cell-free expression
Majumder S, Hsu YY, Andreas MP, Giessen TW, Liu AP✉
Biochemistry (2022) | bioRxiv preprint: 439350
31. Pore structure controls stability and molecular flux in engineered protein cages
Adamson LR*, Tasneem N*, Andreas MP*, Close W, Jenner EN, Szyszka TN, Young RD, Cheah LC, Norman A, MacDermott-Opeskin HI, O’Mara, ML, Sainsbury F, Giessen TW✉, Lau YH✉
Science Advances (2022) | bioRxiv preprint: 428512
2021
30. Triggered reversible disassembly of an engineered protein nanocage
Jones JA*, Cristie-David AS*, Andreas MP, Giessen TW✉
Angewandte Chemie International Edition (2021) | bioRxiv preprint: 440480
29. Large-scale computational discovery and analysis of virus-derived microbial nanocompartments
Andreas MP, Giessen TW✉
Nature Communications (2021) | bioRxiv preprint: 436031
28. Engineered Icosahedral Protein Cage as a Flexible Model System to Study Properties of Multi-Motor Transport of Viral Cargoes Badieyan S, Andreas MP, Giessen TW✉, Cianfrocco MW✉
Biophysical Journal 120 (3), 289a (2021)
27. Exploring in vivo cargo loading of encapsulin nanocompartments
Wiggert JA, Rollins N, Silver PA, Giessen TW✉
Scientific Reports (2021)
2020
26. Engineering spatiotemporal organization and dynamics in synthetic cells
Groaz A, Moghimianavval H, Tavella F, Giessen TW, Vecchiarelli AG, Yang Q, Liu AP✉
WIREs Nanomedicine and Nanobiotechnology (2020)
25. Advances in encapsulin nanocompartment biology and engineering
Jones JA, Giessen TW✉
Biotechnology & Bioengineering (2020)
2019
24. The discovery of six new genes encoding for encapsulin nanocompartments in metagenomes
Tracey J, Coronado M, Giessen TW, Lau, MCY, Silver PA, Ward B✉
Scientific Reports (2019)
23. Engineered inter-species amino acid cross-feeding increases population evenness in a synthetic bacterial consortium
Ziesack M, Gibson T, Oliver JKW, Shumaker AM, Hsu BB, Riglar DT, Giessen TW, DiBenedetto NV, Bry L, Way JC, Silver PA, Gerber GK✉ mSystems (2019) | bioRxiv preprint: 426171
22. Large protein organelles form a new iron sequestration system with high storage capacity
Giessen TW✉, Orlando BJ, Verdegaal AA, Chambers MG, Gardener J, Bell DC, Birrane G, Liao M✉, Silver PA✉
eLife (2019) - Highlighted in: eLife digest - Highlighted in: Cell | bioRxiv preprint: 511345
21. A synthetic system that senses Candida albicans and inhibits virulence factors
Tscherner M, Giessen TW, Markey L, Kumamoto CA, Silver PA✉
ACS Synthetic Biology (2019) | bioRxiv preprint: 342287
2018
20. Prokaryotic nanocompartments form synthetic organelles in a eukaryote
Lau YH*, Giessen TW*, Altenburg WJ, Silver PA✉
Nature Communications (2018) | bioRxiv preprint: 244095
2017
19. Engineering carbon fixation with artificial protein organelles
Giessen TW✉, Silver PA✉
Current Opinion in Biotechnology (2017)
18. Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation
Riglar DT, Giessen TW, Baym M, Kerns SJ, Niederhuber MF, Bronson RT, Kotula JW, Gerber GK, Way JC, Silver PA✉
Nature Biotechnology (2017)
17. Widespread distribution of encapsulin nanocompartments reveals functional diversity
Giessen TW, Silver PA✉
Nature Microbiology (2017) | bioRxiv preprint: 085266
2016
16. Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution
Liu X, Lopez P, Giessen TW, Giles M, Way J, Silver PA✉
Scientific Reports (2016) | bioRxiv preprint: 085233
15. Encapsulins: microbial nanocompartments with applications in biomedicine, nanobiotechnology and materials science
Giessen TW✉
Current Opinion in Chemical Biology (2016)
14. A catalytic nanoreactor based on in vivo encapsulation of multiple enzymes in an engineered protein nanocompartment
Giessen TW, Silver PA✉
ChemBioChem (2016)
13. Converting a natural protein compartment into a nanofactory for the size-constrained synthesis of antimicrobial silver nanoparticles Giessen TW, Silver PA✉
ACS Synthetic Biology (2016)
2015
12. Encapsulation as a strategy for the design of biological compartmentalization
Giessen TW, Silver PA✉
Journal of Molecular Biology (2015)
11. Rational and combinatorial tailoring of bioactive cyclic dipeptides
Giessen TW✉, Marahiel MA
Frontiers in Microbiology (2015)
10. A Synthetic Adenylation‐Domain‐Based tRNA‐Aminoacylation Catalyst
Giessen TW✉, Altegoer F, Nebel AJ, Steinbach RM, Bange G✉, Marahiel MA✉
Angewandte Chemie International Edition (2015)
2014
9. The tRNA-dependent biosynthesis of modified cyclic dipeptides
Giessen TW✉, Marahiel MA✉
International Journal of Molecular Sciences (2014)
2013
8. Insights into the generation of structural diversity in a tRNA-dependent pathway for highly modified bioactive cyclic dipeptides
Giessen TW, von Tesmar AM, Marahiel MA✉
Chemistry & Biology (2013)
7. A tRNA-dependent two-enzyme pathway for the generation of singly and doubly methylated ditryptophan 2, 5-diketopiperazines Giessen TW, von Tesmar AM, Marahiel MA✉
Biochemistry (2013)
6. Two [4Fe-4S] clusters containing radical SAM enzyme SkfB catalyze thioether bond formation during the maturation of the sporulation killing factor
Flühe L, Burghaus O, Wieckowski BM, Giessen TW, Linne U, Marahiel MA✉
Journal of the American Chemical Society (2013)
2012
5. Ribosome-independent biosynthesis of biologically active peptides: Application of synthetic biology to generate structural diversity Giessen TW, Marahiel MA✉
FEBS Letters (2012)
4. Isolation, Structure Elucidation, and Biosynthesis of an Unusual Hydroxamic Acid Ester-Containing Siderophore from Actinosynnema mirum
Giessen TW, Franke KB, Knappe TA, Kraas FI, Bosello M, Xie X, Linne U, Marahiel MA✉
Journal of Natural Products (2012)
3. An enzymatic pathway for the biosynthesis of the formylhydroxyornithine required for rhodochelin iron coordination
Bosello M, Mielcarek A, Giessen TW, Marahiel MA✉
Biochemistry (2012)
2. Exploring the mechanism of lipid transfer during biosynthesis of the acidic lipopeptide antibiotic CDA
Kraas FI, Giessen TW, Marahiel MA✉
FEBS Letters (2012)
2011
1. A four-enzyme pathway for 3, 5-dihydroxy-4-methylanthranilic acid formation and incorporation into the antitumor antibiotic sibiromycin Giessen TW, Kraas FI, Marahiel MA✉
Biochemistry (2011)